3D cell culture on nanofibers
Nanofibers produced through electrospinning are polymer fibers with diameters of hundreds of nanometers and have a high similarity to the extracellular matrix (ECM) of the human body. In particular, it is made of biocompatible and biodegradable polymers and has high cell viability and biocompatibility. These nanofibers of biocompatible/biodegradable polymer materials are in the spotlight as cell supports for three-dimensional cell culture.
Two-dimensional cell culture, in which cells are cultured on glass or transparent polymeric plates, has a different culture environment than in humans, and cell adhesion is different from in the body, limiting cell behavior and growth to tissues and organs. Three-dimensional cell culture technology is actively being developed to overcome the limitations of two-dimensional cell culture and provide cells with a more in-body environment, and nanofibers are recognized as one of the most representative materials as a support for three-dimensional cell culture.
Nanofibers have the following advantages:
High similarity to the extracellular matrix of the human body
Cell-friendly three-dimensional network structure
Various fiber structures, such as alignment and non-alignment
Various fiber diameters; nanofibers with diameters of tens to hundreds of nanometers and microfibers with diameters of tens to tens of micrometers
Additional role of topological cues to cells